Although MeJA might potentiate the interaction, particularly in the case of MYC2 and MYC4, it also occurs in the absence of MeJA treatment. BPMs are substrate adaptors of Cullin3-based E3 ubiquitin ligases that target MYC proteins for proteasome degradation in response to JA (i.e., CUL3BPM3) or other unknown signals (i.e., other CULBPMs). Additionally, competition for cis-regulatory elements with bHLH repressors balances MYC activity (15, 30, 63⇓–65). However, due to the toxicity of MYC expression in yeast, these interactions likely represent only a subset of all possible interactions in vivo. Calculation of the delay timet for a quarter note (crotchet) at the tempo b in bpm. The bpm2,bpm3,bpm5 (bpm235) triple mutant was generated by crossing the corresponding double-mutant homozygous lines. The MYC2 C5025 line was also crossed by Col-0, and F1 segregating progenies were used as controls in the same experiment. Moreover, gene ontology (GO) analysis of up-regulated genes in amiR-bpm showed a statistically significant overrepresentation of JA-related GO terms, such as JA response and wounding (Fig. Remarkably, after purification of ubiquitinated proteins with the p62 resin, the polyubiquitinated-protein smear was lower in the amiR-bpm background compared with the WT. 2 comments . Repression of MYC activity is necessary for resetting JA signaling and to avoid harmful runaway responses, which contribute to plant fitness. Results shown in the graphs are the mean ± SD of measurements of 15 to 20 seedlings. Contact Us. F2 segregating progenies of these crosses were genotyped to obtain homozygous plants for each T-DNA insertion line. Two 35S:MYC2-GFP and 1 35S:MYC3-HA transgenic lines previously reported (75) were crossed by Col-0 or introgressed by direct crossing into the amiR-bpm background (43), and F3 homozygous segregating plants were selected in 40 μM hygromycin plates for the wild-type background or in 40 μM hygromycin and 10 μM BASTA plates for the amiR-bpm background, respectively. Similar to the 35S:MYC2-GFP line, the recombineering MYC2-GFP showed an enhanced stability after CHX treatment (Fig. 7A and SI Appendix, Fig. JA-dependent biological processes are highlighted in red. Add at least two items to compare. Since MYC2 is constitutively expressed from the 35S promoter in the transgenic lines, we also analyzed its degradation rate after inhibition of protein synthesis by cycloheximide (CHX) (Fig. 58). Live Chat. They are characterized by the presence of two protein–protein interaction domains: BTB/POZ (broad complex, tramtrack, bric-a-brac/POX virus, and zinc finger domain) and MATH (Meprin and TRAF homology domain). Sony. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. 2A and SI Appendix, Fig. Improved protein-binding microarrays for the identification of DNA-binding specificities of transcription factors, DNA-binding specificities of plant transcription factors and their potential to define target genes, Rational design of a ligand-based antagonist of jasmonate perception, Two bHLH-type transcription factors, JA-ASSOCIATED MYC2-LIKE2 and JAM3, are transcriptional repressors and affect male fertility, Basic helix-loop-helix transcription factors JASMONATE-ASSOCIATED MYC2-LIKE1 (JAM1), JAM2, and JAM3 are negative regulators of jasmonate responses in, bHLH003, bHLH013 and bHLH017 are new targets of JAZ repressors negatively regulating JA responses, Alternative splicing expands the repertoire of dominant JAZ repressors of jasmonate signaling, JAZ8 lacks a canonical degron and has an EAR motif that mediates transcriptional repression of jasmonate responses in, Negative feedback control of jasmonate signaling by an alternative splice variant of JAZ10, Repression of jasmonate signaling by a non-TIFY JAZ protein in, The MATH-BTB BPM3 and BPM5 subunits of Cullin3-RING E3 ubiquitin ligases target PP2CA and other clade A PP2Cs for degradation, Stability of homologue of Slimb F-box protein is regulated by availability of its substrate, Stability of HIB-Cul3 E3 ligase adaptor HIB is regulated by self-degradation and availability of its substrates, The JA-pathway MYC transcription factors regulate photomorphogenic responses by targeting HY5 gene expression, The ZIM domain mediates homo- and heteromeric interactions between, Rapid screening for improved solubility of small human proteins produced as fusion proteins in, A mutation in VPS15 (PIK3R4) causes a ciliopathy and affects IFT20 release from the, The UPF1 interactome reveals interaction networks between RNA degradation and translation repression factors in, Proceedings of the National Academy of Sciences, Earth, Atmospheric, and Planetary Sciences, ORCID record for Jose Manuel Franco-Zorrilla,,,,, CUL3BPM E3 ubiquitin ligases regulate MYC2, MYC3, and MYC4 stability and JA responses, Inner Workings: Early Mars may have boasted a large ocean and cool climate, Journal Club: New technique builds animal brain–like spontaneity into AI, US racial inequality: A pandemic-scale problem.
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